Reversal of multidrug resistance of hepatocellular carcinoma cells by metformin through inhibiting NF-κB gene transcription

Figure 1 The levels of P-glycoprotein, mdr1 and nuclear factor-κB expression in different cell lines. A and C: The levels of P-gp and NF-κB expression in HepG2 or HepG2/ADM cells were determined by Western blot. The number indicates the ratio of HepG2/ADM cells to HepG2 cells (n = 3, mean ± SD); B and D: The gray intensity images of Figure 1A and Figure 1C, respectively; E: The levels of mdr1 and NF-κB mRNA expression were determined by qRT-PCR. ^bP < 0.01 (n = 3, mean ± SD), compared with hepG2 or LO2 cell line. P-gp: P-glycoprotein; NF-κB: Nuclear factor-κB; ADM: Adriamycin.