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©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
Preserved liver regeneration capacity after partial hepatectomy in rats with non-alcoholic steatohepatitis
David Haldrup, Sara Heebøll, Karen Louise Thomsen, Henning Grønbæk, Department of Hepatology and Gastroenterology, Aarhus University Hospital, Aarhus C DK-8000, Denmark
David Haldrup, Department of Internal Medicine, Randers Regional Hospital, Randers NØ DK-8930, Denmark
Kasper Jarlhelt Andersen, Michelle Meier, Frank Viborg Mortensen, Department of Surgical Gastroenterology, Aarhus University Hospital, Aarhus C DK-8000, Denmark
Jens Randel Nyengaard, Stereology and Electron Microscopy Laboratory, Centre for Stochastic Geometry and Advanced Bioimaging, Aarhus University Hospital, Aarhus C DK-8000, Denmark
Stephen Hamilton-Dutoit, Institute of Pathology, Aarhus University Hospital, Aarhus C DK-8000, Denmark
Author contributions: Haldrup D and Heebøll S performed the majority of the experiments with assistance and supervision by Thomsen KL, Andersen KJ, Meier M and Nyengaard JR; Thomsen KL, Andersen KJ, Meier M, Mortensen FV, Nyengaard JR and Grønbæk H concived and designed the study; Thomsen KL performed the statistical analysis; Hamilton-Dutoit S performed the histological evaluation; all authors contributed to the analysis and interpretation of data; Haldrup D wrote the original manuscript draft; Heebøll S, Thomsen KL, Andersen KJ, Meier M, Nyengaard JR, Mortensen FV, Nyengaard JR, Hamilton-Dutiot S and Grønbæk H critically revised the manuscript for important intellectual content; all authors saw and approved the final manuscript.
Supported by NOVO Nordisk Foundation (grant number 1013267) and Savværksejer Jeppe Juhl og Hustru Ovita Juhls Mindelegat to Grønbæk H; Villum Fonden to Nyengaard JR.
Institutional animal care and use committee statement: All animal work was conducted according to the national guidelines. The Danish Animal Experiments Inspectorate approved the study (2014-15-2934-00997).
Conflict-of-interest statement: To the best of our knowledge there are no competing interests.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: David Haldrup, MD, Department of Hepatology and Gastroenterology, Aarhus University Hospital, Nørrebrogade 44, Aarhus C DK-8000, Denmark. davihald@rm.dk
Telephone: +45-20912152
Received: October 12, 2017
Peer-review started: October 13, 2017
First decision: November 7, 2017
Revised: November 20, 2017
Accepted: December 6, 2017
Article in press: December 6, 2017
Published online: January 27, 2018
Peer-review started: October 13, 2017
First decision: November 7, 2017
Revised: November 20, 2017
Accepted: December 6, 2017
Article in press: December 6, 2017
Published online: January 27, 2018
Core Tip
Core tip: Liver regeneration capacity has been studied in different animal models of non-alcoholic steatohepatitis. This study is the first to use a high fat high cholesterol model which mimic the pathogenesis of human non-alcoholic steatohepatitis better than previous animal models. Liver regeneration capacity was evaluated using: (1) The total number of Ki-67 positive hepatocytes in the caudate lobe, evaluated in a stereology based design; (2) the regenerated protein content to describe the regenerated liver mass; and (3) the plasma concentration of coagulation factors as a marker of liver function. We found a preserved liver regeneration capacity in rats with non-alcoholic steatohepatitis, adding important knowledge to the subject.