Application and prospects of high-throughput screening for in vitro neurogenesis

Figure 1 Main protocols and applications of in vitro neurogenesis. Neurons can be generated in vitro through the differentiation of induced pluripotent stem cells or the transdifferentiation of somatic cells with the aid of transcription factors, growth factors or small molecules[51,131]. With the combination of 3D biomaterials, induced neurons can be applied in various fields, including modeling, drug screening, neural tissue engineering and regenerative medicine[213,232,276]. iPSCs: Induced pluripotent stem cells.