Disulfide-stabilized single-chain antibody-targeted superantigen: Construction of a prokaryotic expression system and its functional analysis

Table 1 Oligonucleotide primers for construction of B3scdsFv-pET22b

Number	Long oligonucleotide sequences
1	5’-GGC CAT ATG GAT GTG AAG CTG GTG-3’
2	5’-ACC TCC ACC TGA ACC GCC TCC ACC GGA GAC AGT GAC CAG-3’
3	5’-CTG GTC ACT GTC TCC GGT GGA GGC GGT TCA GGT GGA-3’
4	5’-CCG GGA TCC GCC CCC TTT AAT TTC CAG CTT TGT-3’
5	5’-ATTGGATCCGGAGGTTCAAGCGAGAAAAGCGAAGAAATA-3’
6	5’-CACGTCGACTTAACTTGTATATAAATATATAGCAATATGCATG-3’

Restriction enzyme is underlined.