Multi-parameter magnetic resonance imaging of zebularine in liver fibrosis treatment and calcineurin/NFAT3 mechanism

Figure 5 Effect of zebularine on anti-fibrotic and fibrotic gene expression in transforming growth factor-β1-activated LX-2. The message RNA levels of the following genes in LX-2 cells were measured before and after transforming growth factor-β1 activation and after zebularine treatment. A: α-smooth muscle actin (α-SMA); B: Type I collagen; C: SEPTIN9; D: PTEN; E: PPAR-γ; F: PTCH1; G: Protein expression of α-SMA in LX-2 cells following zebularine intervention at varying concentrations. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. ^dP < 0.0001. ¹P vs control. ²P vs transforming growth factor-β1. ³P vs transforming growth factor-β1 + zebularine 70 mmol/L. ⁴P vs zebularine 70 mM. α-SMA: α-smooth muscle actin; TGF: Transforming growth factor; CoL1a1: Type I collagen; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.