Effect of acetyl-L-carnitine on hypersensitivity in acute recurrent caerulein-induced pancreatitis and microglial activation along the brain’s pain circuitry

Figure 4 Microglia in the basolateral amygdala were activated only in mice with caerulein-induced pancreatitis. A: Overview of the mouse brain shown at bregma -2.18 mm, interaural 1.62 mm[50,51]. The quantified area, the basolateral amygdala (BLA), is outlined in yellow; B and C: Examples of BLA from naïve control and vehicle treated caerulein- (CAE) induced pancreatitis mice were stained for ionized calcium-binding adaptor molecule 1; D and E: The convex area, the hull region of the microglia (samples outlined in red in B and C), was significantly enlarged and more intensely stained in mice with CAE-induced pancreatitis; F and G: Similarly, the somal area was significantly enlarged in tissue from mice with CAE-induced pancreatitis, though, the intensity of staining was not different. Naïve control n = 4, CAE + VEH n = 5, ^aP < 0.05 Student’s t-test. CAE: Caerulein; VEH: Vehicle.