Depletion of MRPL35 inhibits gastric carcinoma cell proliferation by regulating downstream signaling proteins

Figure 3 The expression of MRPL35 in gastric carcinoma cells. A: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) detection of MRPL35 in gastric carcinoma cell lines (AGS, NCI-N87, MKN-45 and HGC-27); B and C: shCtrl (negative control virus) and shMRPL35 (lentiviral particles of MRPL35) (PSC32114 or PSC32115) infected AGS cells (B) and HGC-27 cells (C) at 72 h with bright and fluorescent micrographs (100 × magnification); D and E: qRT-PCR was used to analyze the expression of MRPL35 in AGS cells (D) and HGC-27 cells (E) infected with shCtrl and shMRPL35; F: Western blot was used to detect the expression of MRPL35 in AGS cells transfected with shCtrl and shMRPL35, and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was used as an internal control. n = 3. ^bP < 0.01. shCtrl: Negative control virus; shMRPL35: Lentiviral particles of MRPL35; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; GFP: Green fluorescent protein.