NIMA related kinase 2 promotes gastric cancer cell proliferation via ERK/MAPK signaling

Figure 4 NIMA related kinase 2 promotes gastric cancer cell viability, proliferation, and cell cycle progression via ERK/MAPK signaling. A and B: Relative mRNA and protein expression of cyclin D1 in cell lines with ERK silencing and NEK2 overexpression. C and D: CCK8 assay showing cell viability in BGC823 and SGC7901 cell lines and negative controls. E: Flow cytometry analysis of cell cycle distribution in BGC823 and SGC7901 cell lines. F: Quantification of the data shown in E (n = 3). G: EdU incorporation assay showing the percentage of cells in S-phase. H: Quantification of the data shown in G (n = 3). NEK2: NIMA related kinase 2.