Inhibitory effects of patchouli alcohol on stress-induced diarrhea-predominant irritable bowel syndrome

Figure 5 Inhibitory effects of patchouli alcohol on EFS-induced nitrergic contractile responses of isolated IBS-D rat colonic longitudinal smooth muscle. A-F: Mechanical recording and line chart of the mechanism and inhibitory effect of PA (100 μmol/L) on nerve-mediated contraction of stress-induced IBS-D rat colon; G: Effect of L-NAME on EFS-induced contraction of control rat colon [control group (A): n = 32, IBS-D group (B): n = 31, L-NAME-treated control group (C): n = 12; ^aP < 0.05 vs control group, ^bP < 0.01 vs control group, ^cP < 0.05 vs group IBS-D; ANOVA]; H: Effect of PA on L-NAME-induced contraction of IBS-D rat colon [L-NAME-treated IBS-D group (E): n = 14, PA + L-NAME-treated IBS-D group (F): n = 15; ^eP < 0.05 vs L-NAME-treated IBS-D group, ^fP < 0.01 vs L-NAME-treated IBS-D group, unpaired t-test]; I: Effect of PA on L-NAME-induced contraction of control rat colon [L-NAME-treated control group: n = 12, PA + L-NAME-treated control group (D): n = 16. ^gP < 0.05 vs L-NAME-treated control group; unpaired t-test]. Data are expressed as mean ± SE. L-NAME: N^ω-nitro-L-arginine methyl ester hydrochloride, EFS: Electrical field stimulation; IBS-D: Irritable bowel syndrome; PA: Patchouli alcohol.