Caffeic acid phenethyl ester up-regulates antioxidant levels in hepatic stellate cell line T6 via an Nrf2-mediated mitogen activated protein kinases pathway

Figure 2 α-SMA and collegen-1 protein expression in hepatic stellate cell-T6 cells. After HSC-T6 cells were treated with 5 μmol/L, 10 μmol/L and 15 μmol/L CAPE for 24 h, indirect immunofluorescence (× 200) analysis of α-SMA protein expression (A) were undertaken. Western blot analysis of α-SMA and collegen-1 protein expression was also performed. Gray levels were normalized against those of the corresponding β-actin and the results are expressed relative to control (B and C). The data are the mean ± SD of three independent experiments. ^aP < 0.05 vs control. CAPE: Caffeic acid phenethyl ester.