Effect of CXCR3/HO-1 genes modified bone marrow mesenchymal stem cells on small bowel transplant rejection

Figure 3 Expression of fluorescence, protein and mRNA of CXCR3 and HO-1 in CXCR3/HO-1 modified BMMSCs. A: Bright field microscopy of CXCR3/HO-1 modified BMMSCs; B: Fluorescent microscopy of CXCR3/HO-1 modified BMMSCs (the positive expression rate of GFP exceeded 85%); C: Expression of CXCR3 protein in CXCR3/HO-1 modified BMMSCs and native BMMSCs; D: The expression of CXCR3 protein in CXCR3/HO-1 modified BMMSCs (0.96 ± 0.14) was significantly higher than that in native BMMSCs (0.25 ± 0.09, P < 0.05); E: The relative expression of CXCR3 mRNA in CXCR3/HO-1 modified BMMSCs (7.69 ± 1.06) was 6.69 times higher than that in native BMMSCs (1.00 ± 0.20, P < 0.05); F: Expression of HO-1 protein in CXCR3/HO-1 modified BMMSCs and native BMMSCs; G: The expression of HO-1 protein in CXCR3/HO-1 modified BMMSCs (0.91 ± 0.081) was significantly higher than that in native BMMSCs (0.305 ± 0.071, P < 0.05); H: The relative expression of HO-1 mRNA in CXCR3/HO-1 modified BMMSCs (8.71 ± 0.51) was 7.71 times higher than that in native BMMSCs (1.00 ± 0.19, P < 0.05). BMMSCs: Bone marrow mesenchymal stem cells; HO-1: Heme oxygenase-1.