Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells

Figure 5 Screening and confirmation of recombinant clones without the kanamycin unit. A: Diagram of the removal of the Kam unit in SW105 E. coli; B: Electrophoretogram of the PCR product according to the recombinant BAC template with the 5arm primer; C: Electrophoretogram of the PCR product according to the recombinant BAC template with different primers; D: Electrophoretogram of the PCR product with BamHI or AflII digestion. M: Marker; OB: Original BAC; RB: Recombinant BAC; DkB: Deleted kanamycin BAC; 5F: Forward primer of the 5arm; 5R: Reverse primer of the 5arm; 3F: Forward primer of the 3arm; 3R: Reverse primer of the 3arm; Cre: Cre primer.