Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells

doi:10.3748/wjg.v23.i2.224

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jan 14, 2017; 23(2): 224-231
Published online Jan 14, 2017. doi: 10.3748/wjg.v23.i2.224

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Figure 4 Screening and confirmation of recombinant clones. A: Diagram of homologous recombination between BAC and GFPCreERT2; B: Electrophoretogram of the PCR product according to the recombinant BAC template with the 3arm primer; C: Electrophoretogram of recombinant Gpm6a BAC digested by BamHI and KpnI; D: Electrophoretogram of recombinant Reelin BAC digested by BamHI and SmaI. M: Marker; OB: Original BAC; RB: Recombinant BAC. BAC: Bacterial artificial chromosome.

Citation: Shi HB, Lou JL, Shi HL, Ren F, Chen Y, Duan ZP. Construction of Gpm6a/Reelin^GFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells. World J Gastroenterol 2017; 23(2): 224-231
URL: https://www.wjgnet.com/1007-9327/full/v23/i2/224.htm
DOI: https://dx.doi.org/10.3748/wjg.v23.i2.224