Catheterization of the gallbladder: A novel mouse model of severe acute cholangitis

Figure 2 Liver injury is significantly facilitated in severe acute cholangitis mouse model. A: Relative expression levels of IL-1β, IL-6 and TNF-α in liver tissues were examined by real-time PCR at 24 h after the last operation (n = 6); B and C: Determination of pro-inflammatory cytokines in serum and bile of mice by ELISA at 48 h after the last operation (n = 6); D and E: The activity of Caspase 3 and the ratio of Bax to Bcl2 protein expression level were examined in different groups (n = 6); F: Histological examination of liver tissues (HE staining × 200, PCNA-IHC staining × 200), lung tissues (HE staining × 200) and kidney tissues (HE staining × 400) in different groups. Asterisk: necrosis. Black arrow: Inflammatory cell infiltration in liver tissue. Red arrow: PCNA-positive hepatocytes. Green arrow: abundant inflammatory cell infiltration in lung tissue. Blue arrow: The cells with vacuolization in kidney. ^aP < 0.05 vs sham group; ^cP < 0.05 vs LPS group; ^eP < 0.05, difference between the two groups.