Activation of AMPK/MnSOD signaling mediates anti-apoptotic effect of hepatitis B virus in hepatoma cells

Figure 3 MnSOD contributes to decreased mitochondrial superoxide and apoptotic cells in HepG2. 215 cells. A: After 6 d, the interference effect of MnSOD siRNA (siMnSOD) was analyzed by Western blot analysis. MnSOD siRNA (siMnSOD) or non-specific siRNA (siCTRL) was transfected into HepG2.215 cells for 12 h before serum depletion; B: After 6 d, cells were harvested for quantification of mitochondrial superoxide anion formation by flow cytometry. In parallel, (C) cell viability and (D) apoptotic cells were separately determined by MTT assay and flow cytometry. ^aP < 0.05 and ^bP < 0.01 vs siCTRL group.