Activation of AMPK/MnSOD signaling mediates anti-apoptotic effect of hepatitis B virus in hepatoma cells

Figure 1 Resistance of HepG2. 215 cells to apoptosis. A: Typical photographs of HepG2 and HepG2.215 cells cultured for 6 d after serum depletion. The data are representative of three independent experiments; B: MTT assay for the viability of cells cultured for 2, 4 and 6 d after serum depletion. Data represent absorbance at 570 nm and are shown as mean ± SD of quadruplicates. ^aP < 0.05 and ^bP < 0.01 vs HepG2 cells group. The data are representative of three independent experiments; C: In parallel experiments, samples were subjected to FITC-Annexin V/propidium iodide staining and the quantitative analysis of apoptotic cells was performed using flow cytometry. Quantification of apoptotic cells is shown as mean ± SD of triplicates.