Recombinant adenovirus containing hyper-interleukin-6 and hepatocyte growth factor ameliorates acute-on-chronic liver failure in rats

Figure 6 Protein levels of Bcl-2, Bax, Caspase-3 and HMGB1 detected by Western blot, revealing the relative protein expression of Bcl-2, Bax, Caspase-3 and HMGB1, and the ratio of Bcl-2/Bax of all treated livers. A: Bcl-2 protein; B: Bax protein; C: the ratio of Bcl-2/Bax; D: Caspase-3 protein; E: HMGB1 protein. A, B, D, E: The Y axis represents the relative protein expression of Bcl-2, Bax Caspase-3 and HMGB1 compared to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for all types of treated liver; C: The Y axis represents the ratio of the relative protein expression of Bcl-2/Bax for all types of treated liver. ^aP < 0.05 vs Ad-HIL-6 treated, ^bP < 0.01 vs control, ^cP < 0.05 vs Ad-HGF treated, ^dP < 0.01 vs Ad-GFP treated. The results are expressed as the mean ± SD, n = 6. Control: Normal liver; Model: ACLF liver; Ad-GFP: pDC315-3FLAG-sv40-EGFP; Ad-HIL-6: pDC315-3FLAG-sv40-Hyper-IL-6-EGFP; Ad-HGF: pDC315-3FLAG-sv40-HGF-EGFP; Ad-HGF-HIL-6: pDC315-3FLAG-sv40-HGF-IRES-Hyper-IL-6-EGFP.