Basic Study
Copyright ©The Author(s) 2015.
World J Gastroenterol. Nov 21, 2015; 21(43): 12381-12391
Published online Nov 21, 2015. doi: 10.3748/wjg.v21.i43.12381
Figure 4
Figure 4 Retinoic acid receptor α inhibits reactive oxygen species-induced apoptosis in vitro. LE540, an inhibitor of RARα, was cocultured with FL83B cells for 24 h, and then the cells were harvested 6 h after H2O2 treatment. A: Expression levels of Bcl-2, cleaved caspase 3 and β-actin were measured by Western blot; B: The number of apoptotic cells was assessed by AV and PI labeling. Mean ± SD, n = 3-5/group, aP < 0.05 vs the DMSO group; bP < 0.05 vs the H2O2 group. RARα: Retinoic acid receptor α; AV: Annexin V; PI: Propidium iodide; FITC: Fluorescein isothiocyanate; ATRA: All-trans retinoic acid.