Increase in apoptosis by combination of metformin with silibinin in human colorectal cancer cells

Figure 4 Combined metformin and silibinin treatment induces COLO 205 cell apoptosis. COLO 205 cells were treated with silibinin and metformin in combination or alone. Apo-Glo assay showing apoptosis of COLO 205 cells treated with silibinin (100 μmol/L) and metformin (10 mmol/Lol/L) in combination (M + S) or alone (A); The protein samples were probed using an antibody against AIF (B) or activated caspase 3 (C). All data are expressed as mean ± SE (n = 6 for each group). ^bP < 0.001 vs Control. Con: Control; Veh: Vehicle; Sili: silibinin; Met: Metformin; M + S: Metformin and silibinin.