Glycogenotic hepatocellular carcinoma with glycogen-ground-glass hepatocytes: A heuristically highly relevant phenotype

Figure 3 Human focal (A, B) and nodular (C, D) hepatic glycogenosis, and more advanced mixed cell populations (E, F) with intrafocal small cell change. A, B: Hepatic vein. Perivenular glycogenolytic (clear cell) focus in an hepatocellular carcinoma (HCC)-bearing liver with hepatitis B virus (HBV)-associated cirrhosis, demonstrated in serial sections with hematoxylin and eosin (A) and periodic acid schiff (PAS) (B)-reaction counterstained with orange G and iron hematoxilin (Tri-PAS). Bar: 100 μm; C, D: Glycogenotic (clear cell) nodule in an HCC-free liver with HBV-associated cirrhosis demonstrated in serial sections with hematoxylin-eosin (C) and Tri-PAS (D). Bar: 100 μm; E, F: Hematoxylin and eosin (E), proliferating cell nuclear antigen (F) immunostaining. Increased cell proliferation (arrows) in a mixed cell focus with less pronounced glycogen storage (not shown) and with intrafocal small cell change in liver with cryptogenic cirrhosis, demonstrated in serial sections. Bar: 50 μm, from Su et al[68].