Side-stream smoking reduces intestinal inflammation and increases expression of tight junction proteins

Table 1 Primer sets used for quantitative reverse transcriptase polymerase chain reaction of mouse large intestine tissue

Gene name	Accession no.	Product size	Direction	Sequence (5’→3’)	Source
IL-6	NM_031168.1	107 bp	Forward	GCTGGTGACAACCACGGCCT	This study
			Reverse	AGCCTCCGACTTGTGAAGTGGT
TNF-α	NM_013693.2	67 bp	Forward	TGGGACAGTGACCTGGACTGT	[58]
			Reverse	TTCGGAAAGCCCATTTGAGT
Claudin 3	NM_009902.4	132 bp	Forward	CAGGGGCAGTCTCTGTGCGAG	This study
			Reverse	GCCGCTGGACCTGGGAATCAAC
Occludin	NM_008756.2	308 bp	Forward	ATGTCCGGCCGATGCTCTC	[58]
			Reverse	TTTGGCTGCTCTTGGGTCTGTAT
ZO-1	NM_009386.2	403 bp	Forward	ACCCGAAACTGATGCTGTGGATAG	[58]
			Reverse	AAATGGCCGGGCAGAACTTGTGTA
ZO-2	AF113005.1	106 bp	Forward	CCCAGCACCAAGCCACCTTTTCA	This study
			Reverse	TCGGTTAGGGCAGACACACTCCC
GAPDH	NM_008084.2	132 bp	Forward	AACTTTGGCATTGTGGAAGG	This study
			Reverse	GGATGCAGGGATGATGTTCT

IL-6: Interleukin-6; TNF-α: Tumor necrosis factor-α; ZO: Zona occludens; GPADH: Glyceraldehyde-3-phosphate dehydrogenase.