Over-expression of uPA increases risk of liver injury in pAAV-HBV transfected mice

Figure 2 Establishment of albumin-tetracycline reverse transcriptional activator and tetO-urokinase plasminogen activator transgenic mice. A: The albumin-tetracycline reverse transcriptional activator (rtTA) unit contains the mouse albumin enhancer/promoter, rtTA coding sequence, and SV40 polyA. The tetO-urokinase plasminogen activator (uPA) unit contains the TRE₂-PminCMV, uPA cDNA, uPA exon11. Arrowheads depict the positions and directions of the polymerase chain reaction (PCR) primers; B: PCR identification of the transgenic founders. 1-9, PCR identification for the nine albumin-rtTA transgenic founder mice; 1-5, PCR identification for the five tetO-uPA transgenic founder mice. CMV: Cytomegalovirus; M: Marker; NC: Negative control; PC: Positive control.