Roux-en-Y gastric bypass promotes expression of PDX-1 and regeneration of β-cells in Goto-Kakizaki rats

Figure 4 Anti-Brdu and -insulin double IHC staining. A: Brdu and insulin double positive cells in the islets or acini (arrowheads), single Brdu positive cells in the acini (thin-tail arrow); B: Brdu and insulin double positive cells in the islets (arrowhead) and single Brdu positive cells in the acini (thin-tail arrow), insulin positive cells in the acini (short-tail arrow); C: Brdu and insulin double positive cells in the ducts (thin-tail arrows) and islets (arrowhead); D: Single Brdu positive cells in the acini (thin-tail arrow); E: No Brdu and insulin double labeling cells in the pancreas of sham-RYGB rats; F: The index of Brdu and insulin double positive cells. (A and C at 400 × magnifications; B and E at 200 × magnifications; D at 1000 × magnifications). ^aP < 0.05 vs sham-RYGB; ^cP < 0.05 vs sham-op group.