Lentivirus-mediated shRNA interference targeting STAT3 inhibits human pancreatic cancer cell invasion

Figure 1 Structure of vectors. A: Lentivirus vector pGCL-GFP containing a CMV driven GFP reporter and a U6 promoter upstream of cloning restriction sites (HpaI and XhoI) to allow the introduction of oligonucleotides encoding shRNAs. The multiple cloning site (MCS) is located between the U6 promoter and CMV. B: The pEGFP-N1 vector expressed green fluorescent protein following transfection into mammalian cells. MCS is located between the immediate early promoter of CMV and the EGFP. ¹Methylated in the DNA provided by CLONTECH.