Depletion of CD4+CD25+ regulatory T cells can promote local immunity to suppress tumor growth in benzo[a]pyrene-induced forestomach carcinoma

Figure 10 PCR analysis of chemokine receptor transcripts in CD4⁺CD25^- and CD4⁺CD25⁺ cells (A). CCR1, CCR4, CCR5, CCR6, CCR7, CCR8, and β-actin mRNA expression of CD4⁺CD25^- cells (lanes 1, 3, 5, 7) and CD4⁺CD25⁺ (lanes 2, 4, 6, 8) in RLNs (lanes 1-4) and PLNs (lanes 5-8) in BaP-only (lanes 1, 2, 5, 6) and control (lanes 3, 4, 7, 8) mice at 32 wk using RT-PCR. Relative expression of chemokine mRNA was compared (B). CD4⁺CD25⁺ cells expressed significantly higher levels of CCR1 (a) and CCR5 (a) mRNA. a: P < 0.05