High expression level of soluble SARS spike protein mediated by adenovirus in HEK293 cells

Figure 3 Effects of tunicamycin on expression of S-protein. HEK293 cells were infected with Ad-S/MS virus for 48h in the presence or absence of tunicamycin (7μg/mL). The media were mixed with Ni-NTA-agarose beads and bound protein was eluted with imidazole elution buffer. The pelleted cells were lysed. After centrifugation, the supernatant with eluted protein from the media was separated on SDS-PAGE gels (7%, v/v). Separated proteins were detected by Western blotting using anti-Myc antibody.