A truncated hepatitis E virus ORF2 protein expressed in tobacco plastids is immunogenic in mice

Figure 2 Transformed plastid genomes revealed by Southern blot analysis. A: Maps of WT and transplastomic genomes showing the positions of KpnI and NcoI sites, and areas to which different probes hybridize. The restriction fragment lengths are given in kb. B: DNA gel blot analysis confirming transgene integration into chloroplast genomes. DNA was digested by KpnI and NcoI. The blots were hybridized with probes targeting the psaB-trnfM arm (P1), trnG-psbC arm (P2), rice psbA promoter (P3) and aadA-coding region (P4). Arrows indicate the sizes of the hybridized DNA fragments in kb.