Cytoskeleton reorganization and ultrastructural damage induced by gliadin in a three-dimensional in vitro model

Figure 2 Phase-contrast micrographs (10×) showing control (A) and gliadin-treated multicellular tumor spheroids of the human colon adenocarcinoma LoVo cell line (B) after 11 d of culture; Scanning electron micrographs: showing the ovoid control spheroids (C) and their very compact, densely organized and tightly packed structure that they can be clearly distinguished from each other; The surface of gliadin-treated spheroids (D) focally interrupted by irregularly distributed holes, and loss of their structural thickness and organization (E).