DNA end binding activity and Ku70/80 heterodimer expression in human colorectal tumor

Figure 1 Representative gel shift experiments on nuclear (A) and cytoplasmic (B) extracts from human colon tissues. Panel A: Normal (n) and pathologic (t) nuclear extracts, obtained from four colorectal cancer (K1-K4) and five adenoma (A1-A5) patients, were analyzed by EMSA as described in "Materials and methods". The positions of Ku band-shifts and of the free probe (FP) are indicated. The binding activity in nuclear extracts from the CaCo-2 cell line (CaCo) has been used as a standard in order to compare the activity in the different gels. Lane P: free probe without protein extract. Panel B: EMSA was performed on pathologic cytoplasmic extracts from colorectal cancer (K1-K6) and adenoma (A1-A9) patients. Lane P: free probe without protein extract.