Characterization of colonic dendritic cells in normal and colitic mice

Figure 2 Colonic DC are myeloid DC which are distributed throughout the colon. Frozen sections of normal colon were stained with MAC-1 and FITC (A), CD11c and Cy3 (B) and counterstained with DAPI. The overlay of the combined images (C) shows that CD11c and MAC-1 co-localized (yellow) consistent with these cells being MAC-1⁺, CD11c⁺ myeloid DC. Tissue from the proximal (D), mid (E), and distal (F) colon of normal animals were immunolabeled with CD11c-Cy.3 to identify DC, cytokeratin-FITC to identify epithelial cells and counterstained with DAPI. DC were distributed throughout the colon. Magnification, ×160. Tissue was analyzed from the colons of 5 C57BL/6-^IL2-/- and 4 C57BL/6 animals.