Effect of Chaiqinchengqi decoction on sarco/endoplasmic reticulum Ca2+-ATPase mRNA expression of pancreatic tissues in acute pancreatitis rats

Figure 1 Scores of pancreatic tissues in three groups (n = 10, mean ± SD). The pathological score of pancreatic tissues was the highest in the AP group (9.2 ± 2.7) and followed by CQCQD group (5.7 ± 1.9) and C group (0.8 ± 1.0), (P < 0.05).

Figure 2 Intracellular [Ca²⁺]_i of pancreatic acini (n = 10, mean ± SD). The fluorescence intensity (FI) of intracellular [Ca²⁺]_i of pancreatic acinar in AP group (138.2 ± 23.1) was higher than C group (111.0 ± 18.4) and CQCQD group (118.7 ± 15.2 , P < 0.05), but there was no statistical difference between the C group and the CQCQD group (P > 0.05).

Figure 3 Agar gel electrophoresis photograph of PCR products of SERCA1 mRNA. The PCR products of sarcoplasmic reticulum Ca²⁺-ATPase (SERCA1) mRNA was 199 bp. M: Marker; PC: Positive control with skeletal muscle; C: Control group; A: Acute pancreatitis group; D: Acute pancreatitis group treated with Chaiqinchengqi decoction.

Figure 4 Ct value of GAPDH and SERCA2 mRNA of RT-PCR. Ct: Cycle threshold; G: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), the internal housekeeping; S: Sarco/endoplasmic reticulum Ca²⁺-ATPase 2 (SERCA2).

Figure 5 Agar gel electrophoresis photograph of PCR products of SERCA2 mRNA. D: Acute pancreatitis group treated with Chaiqinchengqi decoction; A: Acute pancreatitis group; C: Control group; G: Internal housekeeping gene (GAPDH gene); M: Marker.