Basic Study
Copyright ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 28, 2017; 23(28): 5158-5166
Published online Jul 28, 2017. doi: 10.3748/wjg.v23.i28.5158
Single amino acid mutation of SR-BI decreases infectivity of hepatitis C virus derived from cell culture in a cell culture model
Rong Gao, Wei Gao, Gang Xu, Jie Xu, Hao Ren
Rong Gao, Wei Gao, Gang Xu, Jie Xu, Hao Ren, Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai 200433, China
Author contributions: Gao R, Gao W and Xu J performed the experiments; Xu G and Ren H designed the study and contributed to data acquisition, analysis and interpretation; Gao R and Ren H drafted and critically revised the manuscript; all authors approved the final version of the article to be published; Gao R and Gao W contributed equally to this work.
Supported by National Natural Science Foundation of China, No. 31370196; and the National 973 Program, No. 2013CB531601.
Institutional review board statement: The study was approved by the Institutional Review Board of the Second Military Medical University.
Institutional animal care and use committee statement: No animals were used in this experiment.
Conflict-of-interest statement: The authors have no conflicts of interest regarding this article.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Hao Ren, Professor, Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China. renh@smmu.edu.cn
Telephone: +86-21-81870996 Fax: +86-21-81870988
Received: April 13, 2017
Peer-review started: April 18, 2017
First decision: May 16, 2017
Revised: May 24, 2017
Accepted: July 4, 2017
Article in press: July 4, 2017
Published online: July 28, 2017
Core Tip

Core tip: Human class B scavenger receptor I (SR-BI) plays important roles in both host lipid metabolism and the entry of hepatitis C virus (HCV). Single nucleotide polymorphisms (SNPs) in the host genome that affect the virus-host interaction have received increasing attention in recent years. Several SR-BI SNPs have been reported to affect the high-density lipoprotein cholesterol levels in populations carrying SR-BI mutations; however, the impact of SR-BI SNPs on HCV infection has not been studied intensively. Based on our results, the S112F single amino acid mutation in SR-BI inhibited the infectivity of hepatitis C virus derived from cell culture in a cell culture model by downregulating the expression of the SR-BI protein.