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©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 21, 2017; 23(27): 4935-4941
Published online Jul 21, 2017. doi: 10.3748/wjg.v23.i27.4935
Published online Jul 21, 2017. doi: 10.3748/wjg.v23.i27.4935
Human liver chimeric mouse model based on diphtheria toxin-induced liver injury
Xiao-Nan Ren, Rong-Rong Ren, Hua Yang, Bo-Yin Qin, Xiu-Hua Peng, Li-Xiang Chen, Shun Li, Meng-Jiao Yuan, Chao Wang, Xiao-Hui Zhou, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China
Xiao-Hui Zhou, Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, Fudan University, Shanghai 201508, China
Author contributions: Ren XN and Zhou XH designed the experiments; Ren XN, Ren RR and Yang H performed the majority of experiments; Ren XN and Zhou XH analyzed the data; Qin BY, Peng XH, Chen LX, Yuan MJ and Wang C contributed to genotyping; Ren XN and Zhou XH wrote the paper; Li S revised the paper.
Supported by Shanghai Science and Technology Development Foundation Project , No. 12140900300 ; Shanghai Municipal Commission of Health and Family Planning Project , No. 20144Y0073 ; Shanghai Public Health Clinical Center Project , No. 2014M08 ; and National Science and Technology Major Project , No. 2017ZX10304402-001-012 .
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of Shanghai Public Health Clinical Center Affiliated to Fudan University.
Conflict-of-interest statement: No conflicts of interest exist in this study.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Xiao-Hui Zhou, MD, PhD, Professor, Shanghai Public Health Clinical Center, Fudan University, 2901 Caolang Road, Jin Shan District, Shanghai 201508, China. zhouxiaohui@shaphc.org
Telephone: +86-21-37990333 Fax: +86-21-37990333
Received: January 25, 2017
Peer-review started: February 1, 2017
First decision: March 16, 2017
Revised: April 1, 2017
Accepted: June 1, 2017
Article in press: June 1, 2017
Published online: July 21, 2017
Peer-review started: February 1, 2017
First decision: March 16, 2017
Revised: April 1, 2017
Accepted: June 1, 2017
Article in press: June 1, 2017
Published online: July 21, 2017
Core Tip
Core tip: We established a novel liver chimeric mouse model following liver damage caused by intraperitoneal injection of diphtheria toxin (DT), and transplanted human hepatocytes to obtain liver-humanized mice. After 28 d, human albumin was detected in these mice. Human hepatocytes were successfully repopulated in the livers of triple-crossed albumin-cre transgenic mice, inducible DT receptor transgenic mice and severe combined immune deficient-beige mice [i.e., Alb-cre/DTR/SCID-beige (ADSB) mice]. Our inducible mouse model of humanized liver in ADSB mice may have functional applications, such as studies on hepatocyte transplantation, hepatic regeneration and drug metabolism.