Basic Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 14, 2016; 22(46): 10158-10165
Published online Dec 14, 2016. doi: 10.3748/wjg.v22.i46.10158
NADPH oxidase-1 deficiency offers little protection in Salmonella typhimurium-induced typhlitis in mice
Fong-Fong Chu, R Steven Esworthy, James H Doroshow, Binghui Shen
Fong-Fong Chu, Department of Gastroenterology and Hepatology, The First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology, Luoyang 471003, Henan Province, China
Fong-Fong Chu, R Steven Esworthy, Binghui Shen, Department of Cancer Genetics and Epigenetics, Beckman Research Institute of City of Hope, Duarte, CA 91010, United States
James H Doroshow, Center for Cancer Research and Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, MD 20892, United States
Author contributions: Chu FF and Esworthy RS designed the study, performed the experiments, analyzed the data, and drafted the manuscript; Doroshow JH and Shen B edited the manuscript and provided financial support for the studies.
Supported by Federal funds from the National Cancer Institute (NCI) under Contract, No. HHSN261200800001E (to Chu FF); Research reported in this publication included work performed in the Animal Resources Center Core supported by the National Cancer Institute of the National Institutes of Health under award No. P30CA033572.
Institutional animal care and use committee statement: Care and use of mice in this study conformed to NIH (USA) and Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) standards and were performed under protocol 11043 approved by the City of Hope BRI Institutional Animal Care and Use Committee on 1/12/12 and renewed 1/15/14. Animals were bred and reared in the Animal Resources Center at the City of Hope based on standards and guidelines set by the United States Department of Agriculture; approved by the National Institutes of Health, Office for Laboratory Animal Welfare; and accredited by the AAALAC International.
Conflict-of-interest statement: The authors declare no conflicts of interests.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Fong-Fong Chu, PhD, Staff Scientist, Department of Cancer Genetics and Epigenetics, Beckman Research Institute of City of Hope, 1450 E Duarte Road, Duarte, CA 91010, United States. fchu@coh.org
Telephone: +1-626-359811163831
Received: July 22, 2016
Peer-review started: July 25, 2016
First decision: September 20, 2016
Revised: October 9, 2016
Accepted: November 15, 2016
Article in press: November 16, 2016
Published online: December 14, 2016
Core Tip

Core tip: Using Nox1-knockout mice (Nox1-KO), we examined the role of cecum Nox1 in Salmonella typhimurium (S. Tm) infection. Mice were rendered susceptible to infection with oral metronidazole. Four days after S. Tm inoculation, Nox1-KO mice had equal or slightly lower CFU/g cecum contents and equal or slightly less pathology by histological assessment than wild-type (WT) mice. Quantitative real-time PCR measure of mRNA levels for inflammatory cytokines Il-1β and TNF-α were significantly higher in S. Tm treated WT vs untreated mice but not in S. Tm treated Nox1-KO mice. Since Nox1 may have a role in inflammatory bowel disease, treating subjects with Nox1 inhibitors may not make patients vulnerable to pathogens.