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World J Gastroenterol. Aug 7, 2014; 20(29): 10082-10093
Published online Aug 7, 2014. doi: 10.3748/wjg.v20.i29.10082
Published online Aug 7, 2014. doi: 10.3748/wjg.v20.i29.10082
Dihydromyricetin inhibits migration and invasion of hepatoma cells through regulation of MMP-9 expression
Qing-Yu Zhang, Ran Li, Guo-Fang Zeng, Bin Liu, Jie Liu, Yang Shu, Zhong-Kao Liu, Zhi-Dong Qiu, Dong-Jun Wang, Hui-Lai Miao, Ming-Yi Li, Run-Zhi Zhu, Key Laboratory of Hepatic Disease, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China
Dong-Jun Wang, Department of General Surgery, Jiamusi University, Jiamusi 154024, Heilongjiang Province, China
Author contributions: Zhang QY and Li R contributed equally to the work; Miao HL, Li MY and Zhu RZ designed the research; Zhang QY, Li R, Zeng GF, Liu B, Liu J, Shu Y, Liu ZK, Qiu ZD and Wang DJ performed the research and analyzed the data; Zhu RZ wrote the paper.
Supported by The National Natural Science Foundation of China, No. 81041099; and Natural Science Foundation of Guangdong Province, China, No. S2011010003750
Correspondence to: Run-Zhi Zhu, PhD, Key Laboratory of Hepatic Disease, Affiliated Hospital of Guangdong Medical College, No. 57 Renmin Road, Zhanjiang 524001, Guangdong Province, China. hepatolab@163.com
Telephone: +86-759-2387596 Fax: +86-759-2387596
Received: November 26, 2013
Revised: March 4, 2014
Accepted: March 12, 2014
Published online: August 7, 2014
Revised: March 4, 2014
Accepted: March 12, 2014
Published online: August 7, 2014
Core Tip
Core tip: The novel findings of this report are that dihydromyricetin (DHM) can strongly inhibit the migration and invasion (in transwell experiments) of the hepatic cancer cell lines SK-Hep-1 and MHCC97L. Western blot analysis showed that DHM down-regulated the level of matrix metalloproteinase 9 (MMP-9) protein but had little effect on the expression of MMP-2. Further investigation indicated that this reduction of MMP-9 expression underlies the inhibitory effects of DHM on cell migration and invasion and that this decrease was associated with an increase in the total protein levels of PKC-δ and decrease in the protein phosphorylation levels of members of the MAPK signaling pathway.