Research Report
Copyright ©2014 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 14, 2014; 20(46): 17426-17433
Published online Dec 14, 2014. doi: 10.3748/wjg.v20.i46.17426
Lobaplatin inhibits growth of gastric cancer cells by inducing apoptosis
Chu-Yang Yin, Xiao-Lin Lin, Lei Tian, Ming Ye, Xin-Ying Yang, Xiu-Ying Xiao
Chu-Yang Yin, Department of Breast Surgery, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China
Xiao-Lin Lin, Lei Tian, Ming Ye, Xiu-Ying Xiao, Department of Oncology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China
Xin-Ying Yang, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
Author contributions: Xiao XY and Yang XY designed the research; Xiao XY and Yin CY performed the research; Lin XL and Tian L assisted with analysis of the results and revised the manuscript; Xiao XY and Yin CY analyzed the data and wrote the paper.
Supported by National Natural Science Foundation of China, No. 81101648
Correspondence to: Xiu-Ying Xiao, MD, PhD, Associate Professor, Department of Oncology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dongfang Road, Shanghai 200127, China. xiaoxiuying2002@163.com
Telephone: +86-21-68385559 Fax: +86-21-68385559
Received: March 1, 2014
Revised: April 9, 2014
Accepted: July 22, 2014
Published online: December 14, 2014
Abstract

AIM: To assess the anti-cancer effect of lobaplatin on human gastric cancer cells, and to explore the underlying molecular mechanisms.

METHODS: The human gastric cancer cell lines MKN-28, AGS and MKN-45 were used. The cytotoxicity of lobaplatin was detected using an MTS cell proliferation assay. Flow cytometry was used to detect cell apoptosis using Annexin V-FITC Apoptosis Detection Kit. The expression of apoptosis-regulated genes was examined at the protein level using Western blot.

RESULTS: Lobaplatin inhibited the proliferation of human gastric cancer cells and induced apoptosis, which may be associated with the up-regulation of Bax expression, poly(ADP-ribose) polymerase (PARP) cleavage, p53 expression and the reduction of Bcl-2 expression.

CONCLUSION: The cytotoxicity of lobaplatin may be due to its ability of inducing apoptosis of gastric cancer cells, which would support the potential use of lobaplatin for the therapy of gastric cancer.

Keywords: Gastric cancer, Lobaplatin, Apoptosis

Core tip: Gastric cancer is one of the common malignancies and the main cause of death. Although cisplatin had become a primary therapeutic drug in advanced gastric cancer, drug resistance was the leading cause of treatment failure. To find an effective treatment is particularly urgent and important. Lobaplatin has been investigated in patients with advanced solid tumors, yet it has not been comprehensively studied in gastric cancer cells. In this study, we found the cytotoxicity and the apoptosis promoting effect of lobaplatin, which can provide a new basis for its clinical application in gastric cancer.