Stec-Michalska K, Malicki S, Michalski B, Peczek L, Wisniewska-Jarosinska M, Nawrot B. Gastric ghrelin in relation to gender, stomach topography and Helicobacter pylori in dyspeptic patients. World J Gastroenterol 2009; 15(43): 5409-5417 [PMID: 19916170 DOI: 10.3748/wjg.15.5409]
Corresponding Author of This Article
Barbara Nawrot, PhD, Professor of Chemistry, Head, Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies of the Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, Poland. bnawrot@bio.cbmm.lodz.pl
Article-Type of This Article
Original Article
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Nov 21, 2009; 15(43): 5409-5417 Published online Nov 21, 2009. doi: 10.3748/wjg.15.5409
Gastric ghrelin in relation to gender, stomach topography and Helicobacter pylori in dyspeptic patients
Krystyna Stec-Michalska, Sebastian Malicki, Blazej Michalski, Lukasz Peczek, Maria Wisniewska-Jarosinska, Barbara Nawrot
Krystyna Stec-Michalska, Maria Wisniewska-Jarosinska, Department of Gastroenterology and Internal Diseases, Medical University of Lodz, Plac Hallera 1, 90-647 Lodz, Poland
Sebastian Malicki, Lukasz Peczek, Barbara Nawrot, Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies of the Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, Poland
Blazej Michalski, II Department of Cardiology, Medical University of Lodz, 1/5 Kniaziewicza St, 91-347 Lodz, Poland
Author contributions: Stec-Michalska K conceived of the study, screened the patients, collected the biopsy specimens and helped to draft the manuscript; Malicki S carried out the immunoassay; Michalski B carried out the multiplex PCR and analyzed bacterial cytotoxicity; Peczek L helped to adjust the immunoassay and performed the statistical analysis; Wisniewska-Jarosinska M performed histopathological evaluation of biopsy specimens; Nawrot B participated in study design, interpreted the data and wrote the final version of the manuscript.
Supported by The Ministry of Science and Higher Education in Poland project N 402 307336 for 2009-2011 to Nawrot B and No. 2 P05B 117 28 of Medical University of Lodz, Poland (to Stec-Michalska K 2008)
Correspondence to: Barbara Nawrot, PhD, Professor of Chemistry, Head, Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies of the Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, Poland. bnawrot@bio.cbmm.lodz.pl
Telephone: +48-42-6816970 Fax: +48-42-6815483
Received: August 1, 2009 Revised: August 26, 2009 Accepted: September 2, 2009 Published online: November 21, 2009
Abstract
AIM: To investigate the level of gastric ghrelin in stomach mucosa of dyspeptic patients in relation to Helicobacter pylori (H pylori) infection, bacterial cytotoxicity, topography and gender.
METHODS: The study comprised 40 premenopausal women (19 H pylori positive) and 48 men (17 H pylori positive) with functional dyspepsia. All gastric biopsy specimens revealed normal mucosa or non-atrophic gastritis. Gastric ghrelin concentration was determined by Enzyme linked immunosorbent assay. The cagA and vacA strains of bacterial DNA were identified by multiplex polymerase chain reaction.
RESULTS: In general, infection with H pylori caused an increase in gastric ghrelin level regardless of gender and stomach topography. Significantly more hormone was present in both, non-infected and H pylori positive female samples, as compared to males. The distribution of bacterial strains showed cagA(+) vacA s1m1 and cagA(-) vacA s2m2 genotypes as the most common infections in the studied population. A tendency to higher ghrelin levels was observed in less cytotoxic (cagA negative) strain-containing specimens from the antrum and corpus of both gender groups (without statistical significance).
CONCLUSION: An increase in gastric ghrelin levels at the stage of non-atrophic gastritis in H pylori positive patients, especially in those infected with cagA(-) strains, can exert a gastroprotective effect.
