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©2008 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Oct 14, 2008; 14(38): 5857-5867
Published online Oct 14, 2008. doi: 10.3748/wjg.14.5857
Oxidative stress disturbs energy metabolism of mitochondria in ethanol-induced gastric mucosa injury
Jin-Shui Pan, Shao-Zhen He, Hong-Zhi Xu, Xiao-Juan Zhan, Xiao-Ning Yang, Hong-Min Xiao, Hua-Xiu Shi, Jian-Lin Ren
Jin-Shui Pan, Shao-Zhen He, Hong-Zhi Xu, Xiao-Juan Zhan, Xiao-Ning Yang, Hong-Min Xiao, Hua-Xiu Shi, Jian-Lin Ren, Division of Gastroenterology, Zhongshan Hospital, Xiamen University, Xiamen 361004, Fujian Province, China
Jin-Shui Pan, Hong-Zhi Xu, Xiao-Juan Zhan, Xiao-Ning Yang, Hong-Min Xiao, Hua-Xiu Shi, Jian-Lin Ren, Gastroenterology Institute of Xiamen University, Xiamen 361004, Fujian Province, China
Jin-Shui Pan, Hong-Zhi Xu, Xiao-Juan Zhan, Xiao-Ning Yang, Hong-Min Xiao, Hua-Xiu Shi, Jian-Lin Ren, Gastroenterology Center of Xiamen, Xiamen 361004, Fujian Province, China
Shao-Zhen He, Intensive Care Unit, the First Hospital of Zhangzhou, Zhangzhou 363000, Fujian Province, China
Author contributions: Pan JS and Ren JL designed the research; Pan JS, He SZ, Xu HZ, Zhan XJ, Yang XN, Xiao HM and Shi HX performed the research; He SZ, Zhan XJ and Yang XN analyzed the data; Pan JS and Ren JL wrote the paper.
Supported by National Natural Science Foundation of China, No. 30750013; Natural Science Foundation of Fujian Province, No. X0650091
Correspondence to: Jian-Lin Ren, PhD, MD, Professor, Division of Gastroenterology, Zhongshan Hospital, Xiamen University; Gastroenterology Institute of Xiamen University; Gastroenterology Center of Xiamen, Xiamen 361004, Fujian Province, China. jianlinr@msn.com
Telephone: +86-0592-2993170 Fax: +86-0592-2993170
Received: August 2, 2008
Revised: September 20, 2008
Accepted: September 27, 2008
Published online: October 14, 2008
AIM: To study the role of mitochondrial energy disorder in the pathogenesis of ethanol-induced gastric mucosa injury.
METHODS: Wistar rats were used in this study. A gastric mucosal injury model was established by giving the rats alcohol. Gross and microscopic appearance of gastric mucosa and ultrastructure of mitochondria were evaluated. Malondiadehyde (MDA) in gastric mucosa was measured with thiobarbituric acid. Expression of ATP synthase (ATPase) subunits 6 and 8 in mitochondrial DNA (mtDNA) was determined by reverse transcription polymerase chain reaction (RT-PCR).
RESULTS: The gastric mucosal lesion index was correlated with the MDA content in gastric mucosa. As the concentration of ethanol was elevated and the exposure time to ethanol was extended, the content of MDA in gastric mucosa increased and the extent of damage aggravated. The ultrastructure of mitochondria was positively related to the ethanol concentration and exposure time. The expression of mtDNA ATPase subunits 6 and 8 mRNA declined with the increasing MDA content in gastric mucosa after gavage with ethanol.
CONCLUSION: Ethanol-induced gastric mucosa injury is related to oxidative stress, which disturbs energy metabolism of mitochondria and plays a critical role in the pathogenesis of ethanol-induced gastric mucosa injury.
