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World J Gastroenterol. Apr 14, 2006; 12(14): 2280-2283
Published online Apr 14, 2006. doi: 10.3748/wjg.v12.i14.2280
Antiviral effect of Chinese medicine jiaweisinisan in hepatitis B virus transgenic mice
Xiao-Yin Chen, Guang-Dong Tong, Fang Xia
Xiao-Yin Chen, Fang Xia, Department of Traditional Chinese Medicine, Medical College, Jinan University, Guangzhou 510632, Guangdong Province, China
Guang-Dong Tong, Department of Gastroenterology, Shenzhen Traditional Chinese Medical Hospital, Shenzhen 518000, Guangdong Province, China
Supported by the National Natural Science Foundation of China, No. 30000217 and the Natural Science Foundation of Guangdong Province, No. 000359 and No. 2005B30101012
Correspondence to: Professor Xiao-Yin Chen, Department of Traditional Chinese Medicine, Medical College, Jinan University, 601 Huangpu Road, Guangzhou 510632, Guangdong Province, China. tchenxiaoyin@jnu.edu.cn
Telephone: +86-20-85226410
Received: September 23, 2005
Revised: October 10, 2005
Accepted: October 26, 2005
Published online: April 14, 2006
Abstract

AIM: To study the antiviral effect of Chinese medicine jiaweisinisan (JWSNS) on hepatitis B virus (HBV) infection in transgenic mice (TGM).

METHODS: Twenty two 6-8 wk old HBV TGM in the third generation were divided into TGM control group and TGM treated group randomly. The normal control group included ten normal BC 57L/6 mice at the same age. The mice in treated group were administrated with JWSNS at the concentration of 4 g/mL and the dosage of 50 g/kg per d for 30 d, while the mice in TGM control group and normal control group were administrated with normal saline at the same dosage and the same time. Polymerase chain reaction (PCR) was used to assess the contents of HBV DNA in serum of HBV TGM before and after treatments, whereas blot hybridization was utilized to measure the contents of HBV DNA in the liver of both HBV TGM and normal BC 57L/6 mice.

RESULTS: The levels of serum HBV DNA in TGM treated group were remarkably decreased after the treatment of JWSNS (7.662 ± 0.78 vs 5.22 ± 3.14, P < 0.05), while there was no obvious change after administration of normal saline in TGM control group (7.125 ± 4.26 vs 8.932 ± 5.12, P > 0.05). The OD values of HBV DNA in the livers of the mice in TGM treated group were significantly lower than those of TGM control group (0.274 ± 0.096 vs 0.432 ± 0.119, P < 0.01).

CONCLUSION: JWSNS exerts suppressive effects on HBV DNA in the serum and liver of TGM.

Keywords: Traditional Chinese herbs, Hepatitis B virus, Transgenic mice, PCR, Blot hybridization, Chronic hepatitis B