Brief Reports
Copyright ©The Author(s) 2005. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 14, 2005; 11(34): 5358-5361
Published online Sep 14, 2005. doi: 10.3748/wjg.v11.i34.5358
Effect of indomethacin on electrical field stimulation-induced contractions of isolated transverse and longitudinal rat gastric fundus strips
Salimeh Afshin, Mansoor Keshavarz, Mahmood Salami, Fatemeh Mirershadi, Bijan Djahanguiri
Salimeh Afshin, Mansoor Keshavarz, Fatemeh Mirershadi, Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Mahmood Salami, Department of Physiology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran
Bijan Djahanguiri, Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Co-first-authors: Salimeh Afshin and Mansoor Keshavarz
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Mansoor Keshavarz, Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. mkeshavarz@tums.ac.ir
Telephone: +98-21-641-9484 Fax: +98-21-641-9484
Received: December 14, 2005
Revised: January 1, 2005
Accepted: January 5, 2005
Published online: September 14, 2005
Abstract

AIM: To study the effects of indomethacin on the isolated transverse and longitudinal rat gastric fundus strips.

METHODS: The strips were suspended in an organ bath containing oxygenated Krebs solution, and contractile responses to electrical field stimulation were recorded on a physiograph in an isotonic manner after administration of cumulative concentrations of indomethacin. The effects of indomethacin on the strips pretreated with KATP channel modulators, diazoxide and glybenclamide were studied.

RESULTS: Treatment of the transverse strips with indomethacin resulted in a concentration-dependent inhibitory response. In longitudinal strips, biphasic responses were seen, which included a stimulatory response at low concentrations of indomethacin, followed by an inhibitory response at higher concentrations. Diazoxide pre-treatment inhibited the stimulatory response of longitudinal strips. Glybenclamide pre-treatment not only blocked inhibitory effect of the low concentrations of indomethacin on transverse strips, but also increased the amplitude of contractions. Moreover, the drug decreased the amplitude of contractions in longitudinal strips.

CONCLUSION: Responses of the isolated longitudinal and transverse rat gastric fundus strips to indomethacin are not similar, and are influenced by KATP channel modulators.

Keywords: Gastric fundus, KATP channel, Indomethacin, Diazoxide, Glybenclamide