Jiang YA, Luo HS, Fan LF, Jiang CQ, Chen WJ. Effect of antisense oligodeoxynucleotide of telomerase RNA on telomerase activity and cell apoptosis in human colon cancer. World J Gastroenterol 2004; 10(3): 443-445 [PMID: 14760776 DOI: 10.3748/wjg.v10.i3.443]
Corresponding Author of This Article
Ying-An Jiang, Central Hospital of Huangshi City, 43 Wuhan Road, Huangshi 435000, Hubei Province, China. weijin@hs.hb.cninfo.net
Article-Type of This Article
Brief Reports
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Ying-An Jiang, He-Sheng Luo, Department of Gastroenterology, Renming Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
Li-Fang Fan, Department of Pathology, Medical College of Wuhan University, Wuhan 430071, Hubei Province, China
Chong-Qing Jiang, Department of General Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China Wei-Jin Chen, Department of Medical Information, Central Hospital of Huangshi City, Huangshi 435000, Hubei Province, China
Supported by the Science and Technology Research Project of Hubei Province, No. 2002AA301C72
Correspondence to: Ying-An Jiang, Central Hospital of Huangshi City, 43 Wuhan Road, Huangshi 435000, Hubei Province, China. weijin@hs.hb.cninfo.net
Telephone: +86-714-6283783 Fax: +86-714-6233931
Received: October 12, 2003 Revised: November 4, 2003 Accepted: November 15, 2003 Published online: February 1, 2004
Abstract
AIM: To explore the effect of antisense oligodeoxynucleotide (As-ODN) of telomerase RNA on telomerase activity and cell apoptosis in human colon cancer.
METHODS: As-ODN was transfected into SW480 cells by liposomal transfection reagent. Telomerase activity of SW480 cells was examined by telomeric repeat amplification protocol (TRAP) and enzyme-linked immunosorbent assay (ELISA). Apoptosis was analyzed by morphology and flow cytometry.
RESULTS: The telomerase activity in SW480 cells transfected with 1.0 μmol/L of As-ODN for 2-5 days, was significantly decreased in a time-dependent manner, and the cells underwent apoptosis. The missense ODN (Ms-ODN) and the control group transfected with SW480 cells did not show these changes.
CONCLUSION: As-ODN can specifically inhibit the telomerase activity of SW480 cells and induce apoptosis.
