Viral Hepatitis
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 1, 2004; 10(3): 385-388
Published online Feb 1, 2004. doi: 10.3748/wjg.v10.i3.385
Expression of platelet-derived growth factor-BB in liver tissues of patients with chronic hepatitis B
Song-Mei Lou, You-Ming Li, Kai-Ming Wang, Wei-Min Cai, Hong-Lei Weng
Song-Mei Lou, Department of Gastroenterology, Hangzhou First People’s Hospital, Hangzhou 310006, Zhejiang Province, China
You-Ming Li, Kai-Ming Wang, Wei-Min Cai, Hong-Lei Weng, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Song-Mei Lou, Department of Gastroenterology, Hangzhou First People’s Hospital, Hangzhou 310006, Zhejiang Province, China. lousm_72@hotmail.com
Telephone: +86-571-87065701-2184
Received: May 11, 2003
Revised: June 28, 2003
Accepted: July 5, 2003
Published online: February 1, 2004
Abstract

AIM: To study the relationship between expression of platelet-derived growth factor-BB (PDGF-BB) and fibrogenesis in chronic hepatitis B.

METHODS: Hepatic tissues from 43 patients with chronic hepatitis B were embedded in paraffin. The sections were stained with HE and picric acid-sirius red to determine inflammatory activity and fibrosis stages. PDGF-BB expression was detected by immunohistochemistry and assessed semiquantatively. Levels of serum hyaluronic acid (HA), pro-collagen III (PCIII), collagen IV (IV-C) and laminin (LN) were examined by radioimmunoassay (RIA).

RESULTS: The expression level of PDGF-BB was found to be positively correlated with inflammatory activity, fibrosis stage and grade of histological findings (τ = 0.58, 0.55, 0.55, P < 0.01). The positive correlation was also observed between tissue level of PDGF-BB expression and contents of HA, PCIII, IV-C and LN in the circulation (r = 0.52, 0.32, 0.40, 0.33, P < 0.05).

CONCLUSION: PDGF-BB may play some role in the development and progression of liver fibrosis.

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