Brief Reports
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 1, 2004; 10(21): 3185-3187
Published online Nov 1, 2004. doi: 10.3748/wjg.v10.i21.3185
Changes of cytosolic [Ca2+]i in neutrophils in pancreatic microcirculation of rats with caerulein-induced acute pancreatitis under fluid shear stress
Zong-Guang Zhou, You-Qin Chen, Xu-Bao Liu, Wei-Ming Hu, Bo-Le Tian, Huai-Qing Chen
Zong-Guang Zhou, You-Qin Chen, Xu-Bao Liu, Wei-Ming Hu, Bo-Le Tian, Department of General Surgery & Institute of Gastroenteric Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
Huai-Qing Chen, Institute of Biomedical Engineering, West China Center of Medical Sciences, Sichuan University, Chengdu 610041, Sichuan Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 39770722 and the Key Project of National Outstanding Youth Foundation of China, No. 39925032
Correspondence to: Professor Zong-Guang Zhou, Department of General Surgery & Institute of Gastroenteric Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China. zhou767@21cn.com
Telephone: +86-28-85422484 Fax: +86-28-85422484
Received: March 27, 2004
Revised: March 29, 2004
Accepted: April 5, 2004
Published online: November 1, 2004
Abstract

AIM: To investigate the fluid shear stress induced changes of [Ca2+]i in neutrophils in pancreatic microcirculation of experimental acute pancreatitis (AP).

METHODS: Wistar rats (n = 36) were randomized into three groups. A model of AP was established by subcutaneous injection of caerulein. Low-shear 30 viscometer was used to provide steady fluid shear stress on separated neutrophils. The mean fluorescent intensity tested by flow cytometry was used as the indication of [Ca2+]i quantity.

RESULTS: Under steady shear, cytosolic [Ca2+]i showed biphasic changes. The shear rate changed from low to high, [Ca2+]i in different groups decreased slightly and then increased gradually to a high level (P < 0.05). A close correlation was observed between the cytosolic [Ca2+]i level and the alteration of fluid shear stress in regional microcirculation of AP.

CONCLUSION: The increase of [Ca2+]i is highly related to the activation of neutrophils, which contributes to neutrophil adhesion to endothelium in the early phase of AP. The effect of fluid shear stress on [Ca2+]i may play a crucial role in pancreatic microcirculatory failure of AP.

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