Basic Research
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 15, 2004; 10(20): 3021-3025
Published online Oct 15, 2004. doi: 10.3748/wjg.v10.i20.3021
Adenoviral transfer of human interleukin-10 gene in lethal pancreatitis
Zi-Qian Chen, Yao-Qing Tang, Yi Zhang, Zhi-Hong Jiang, En-Qiang Mao, Wei-Guo Zou, Ruo-Qing Lei, Tian-Quan Han, Sheng-Dao Zhang
Zi-Qian Chen, Yao-Qing Tang, Yi Zhang, Zhi-Hong Jiang, En-Qiang Mao, Ruo-Qing Lei, Tian-Quan Han, Sheng-Dao Zhang, Department of Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China
Wei-Guo Zou, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Author contributions: All authors contributed equally to the work.
Supported by Science and Technology Committee of Shanghai Municipal Government, No. 00419019
Correspondence to: Sheng-Dao Zhang, Department of Surgery, Affiliated Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China. chenziq@hotmail.com
Telephone: +86-21-64370045 Ext. 611002
Received: January 15, 2004
Revised: January 23, 2004
Accepted: February 24, 2004
Published online: October 15, 2004
Abstract

AIM: To evaluate the therapeutic effect of adenoviral-vector-delivered human interleukin-10 (hIL-10) gene on severe acute pancreatitis (SAP) rats.

METHODS: Healthy Sprague-Dawley (SD) rats were intraperitoneally injected with adenoviral IL-10 gene (AdvhIL-10), empty vector (Adv0) or PBS solution. Blood, liver, pancreas and lung were harvested on the second day to examine hIL-10 level by ELISA and serum amylase by enzymatic assay. A SAP model was induced by retrograde injection of sodium taurocholate through pancreatic duct. SAP rats were then administered with AdvhIL-10, Adv0 and PBS solution by a single intraperitoneal injection 20 min after SAP induction. In addition to serum amylase assay, levels of hIL-10 and tumor necrosis factor-α (TNF-α ) were detected by RT-PCR, ELISA and histological study. The mortality rate was studied and analyzed by Kaplan–Meier and log rank analysis.

RESULTS: The levels of hIL-10 in the pancreas, liver and lung of healthy rats increased significantly after AdvhIL-10 injection (1.42 ng/g in liver, 0.91 ng/g in pancreas); while there was no significant change of hIL-10 in the other two control groups. The concentration of hIL-10 was increased significantly in the SAP rats after AdvhIL-10 injection (1.68 ng/g in liver, 1.12 ng/g in pancreas) compared to the other two SAP groups with blank vector or PBS treatment (P < 0.05). The serum amylase levels remained normal in the AdvhIL-10 transfected healthy rats. However, the serum amylase level was significantly elevated in the other two control SAP rats. In contrast, serum amylase was down-regulated in the AdvhIL-10 treated SAP groups. The TNF-α expression in the AdvhIL-10 treated SAP rats was significantly lower compared to the other two control SAP groups. The pathohistological changes in the AdvhIL-10 treated group were better than those in the other two control groups. Furthermore, the mortality of the AdvhIL-10 treated group was significantly reduced compared to the other two control groups (P < 0.05).

CONCLUSION: Adenoviral hIL-10 gene can significantly attenuate the severity of SAP rats, and can be used in the treatment of acute inflammation process.

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