Histone acetylation regulates p21WAF1 expression in human colon cancer cell lines

doi:10.3748/wjg.v10.i18.2643

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Sep 15, 2004 (publication date) through May 6, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Colorectal Cancer

World J Gastroenterol. Sep 15, 2004; 10(18): 2643-2646
Published online Sep 15, 2004. doi: 10.3748/wjg.v10.i18.2643

Histone acetylation regulates p21^WAF1 expression in human colon cancer cell lines

Ying-Xuan Chen, Jing-Yuan Fang, Hong-Yin Zhu, Rong Lu, Zhong-Hua Cheng, De-Kai Qiu

Ying-Xuan Chen, Jing-Yuan Fang, Hong-Yin Zhu, Rong Lu, Zhong-Hua Cheng, De-Kai Qiu, Renji Hospital, Shanghai Second Medical University, Shanghai Institute of Digestive Disease, Shanghai 200001, China

Author contributions: All authors contributed equally to the work.

Supported by the National Natural Science Foundation of China, No. 30170413 and Foundation for the Authors of National Excellent Doctoral Dissertation of China, No. 199946 and Foundation of Shanghai Education Committee, No. 02SG45

Correspondence to: Dr. Jing-Yuan Fang, Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China. jingyuanfang@yahoo.com

Telephone: +86-21-63200874 Fax: +86-21-63266027

Received: February 2, 2004
Revised: February 11, 2004
Accepted: February 24, 2004
Published online: September 15, 2004

Abstract

AIM: To investigate the effect of histone acetylation on regulation of p21^WAF1 gene expression in human colon cancer cell lines.

METHODS: Two cell lines, Colo-320 and SW1116 were treated with either trichostatin or sodium butyrate. Expressions of p21^WAF1 mRNA and protein were detected by real-time RT-PCR and Western blotting, respectively. Acetylation of two regions of p21^WAF1 gene-associated histones and total cellular histones were examined by chromatin immunoprecipitation assay and Western blotting.

RESULTS: Trichostatin or sodium butyrate re-activated p21^WAF1 transcription resulted in up-regulated p21^WAF1 protein level in colon cancer cell lines. Those effects were accompanied by an accumulation of acetylated histones in total cellular chromatin and p21^WAF1 gene-associated region of chromatin.

CONCLUSION: Histone acetylation regulates p21^WAF1 expression in human colon cancer cell lines, Colo-320 and SW1116.

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