Xu HT, Zhao YF, Lian ZX, Fan BL, Zhao ZH, Yu SY, Dai YP, Wang LL, Niu HL, Li N, Hammarström L, Borén T, Sjöström R. Effects of fucosylated milk of goat and mouse on Helicobacter pylori binding to Lewis b antigen. World J Gastroenterol 2004; 10(14): 2063-2066 [PMID: 15237435 DOI: 10.3748/wjg.v10.i14.2063]
Corresponding Author of This Article
Professor Ning Li, State Key Laboratories for Agrobiotechnology, China Agriculture University, Beijing 100094, China. ninglbau@public3.bta.net.cn
Article-Type of This Article
H Pylori
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World J Gastroenterol. Jul 15, 2004; 10(14): 2063-2066 Published online Jul 15, 2004. doi: 10.3748/wjg.v10.i14.2063
Effects of fucosylated milk of goat and mouse on Helicobacter pylori binding to Lewis b antigen
Hong-Tao Xu, Yao-Feng Zhao, Zheng-Xing Lian, Bao-Liang Fan, Zhi-Hui Zhao, Shu-Yang Yu, Yun-Ping Dai, Li-Li Wang, Hui-Ling Niu, Ning Li, Lennart Hammarström, Thomas Borén, Rolf Sjöström
Hong-Tao Xu, Zhi-Hui Zhao, Shu-Yang Yu, Yun-Ping Dai, Li-Li Wang, Hui-Ling Niu, Ning Li, State Key Laboratories for Agrobiotechnology, China Agriculture University, Beijing 100094, China
Yao-Feng Zhao, Lennart Hammarström, Center for Biotechnology, Karolinska Institute, Sweden
Zheng-Xing Lian, College of Animal Science and Technology, China Agriculture University, Beijing 100094, China
Bao-Liang Fan, Bio-tech Research Center of Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China
Thomas Borén, Department of Odontology and Oral Microbiology, Umeå University, Sweden
Rolf Sjöström, Department of Odontology, Umeå University, Sweden
Author contributions: All authors contributed equally to the work.
Correspondence to: Professor Ning Li, State Key Laboratories for Agrobiotechnology, China Agriculture University, Beijing 100094, China. ninglbau@public3.bta.net.cn
Telephone: +86-10-62893323 Fax: +86-10-62893904
Received: December 23, 2003 Revised: December 28, 2003 Accepted: January 8, 2004 Published online: July 15, 2004
Abstract
AIM: To evaluate the effects of animal milk containing fucosylated antigens on Helicobacter pylori (H pylori) binding to Lewis b antigen.
METHODS: A mammary gland expression vector containing human α1-3/4-fucosyltransferase cDNA sequences was constructed. Transient expression of human α1-3/4-fucosyltransferase cDNA in goat mammary cell and establishment of transgenic mice were performed. The adhesion inhibitory properties of milk samples were analyzed by using H pylori.
RESULTS: Goat milk samples were found to inhibit bacterial binding to Lewis b antigen. The highest inhibition was observed 42 h after injection of the plasmid. The binding activity of H pylori to Lewis b antigen reduced mostly, by 83%, however milk samples from transgenic mice did not inhibit H pylori binding to Lewis b antigen.
CONCLUSION: The use of “humanized” animal milk produced by the transgenic introduction of fucosylated antigen can perhaps provide an alternative therapy and preventive measure for H pylori infection.
