Yu ZW, Zhao P, Liu M, Dong XS, Tao J, Yao XQ, Yin XH, Li Y, Fu SB. Reversal of 5-flouroucial resistance by adenovirus-mediated transfer of wild-type p53 gene in multidrug-resiatant human colon carcinoma LoVo/5-FU cells. World J Gastroenterol 2004; 10(13): 1979-1983 [PMID: 15222051 DOI: 10.3748/wjg.v10.i13.1979]
Corresponding Author of This Article
Zhi-Wei Yu, Department of Abdominal Surgery, the Third Affiliated Hospital of Harbin Medical University, Harbin 150040, Heilongjiang Province, China. yuzhiwei1974@163.com
Article-Type of This Article
Brief Reports
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Jul 1, 2004; 10(13): 1979-1983 Published online Jul 1, 2004. doi: 10.3748/wjg.v10.i13.1979
Reversal of 5-flouroucial resistance by adenovirus-mediated transfer of wild-type p53 gene in multidrug-resiatant human colon carcinoma LoVo/5-FU cells
Zhi-Wei Yu, Peng Zhao, Ming Liu, Xin-Shu Dong, Ji Tao, Xue-Qin Yao, Xin-Hua Yin, Yu Li, Song-Bin Fu
Zhi-Wei Yu, Peng Zhao, Ming Liu, Xin-Shu Dong, Department of Abdominal Surgery, the Third Affiliated Hospital of Harbin Medical University, Harbin 150040, Heilongjiang Province, China
Ji Tao, Department of General Surgery, The fifth Hospital of Harbin Medical University, Harbin 150040, Heilongjiang Province, China
Xue-Qin Yao, Department of General Surgery, Nanfang Hospital, Guangzhou 510515, Guangdong Province, China
Xin-Hua Yin, Department of Geriatrics, the Second Affiliated Hospital of Harbin Medical University, Harbin 150096, Heilongjiang Provinece, China
Yu Li, Song-Bin Fu, Department of Molecular Biology, Harbin Medical University, Harbin 150057, Heilongjiang Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Zhi-Wei Yu, Department of Abdominal Surgery, the Third Affiliated Hospital of Harbin Medical University, Harbin 150040, Heilongjiang Province, China. yuzhiwei1974@163.com
Telephone: +86-451-6677580-2146
Received: July 4, 2003 Revised: August 21, 2003 Accepted: August 28, 2003 Published online: July 1, 2004
Abstract
AIM: To observe the reversal effects of wide-type p53 gene on multi-drug resistance to 5-FU (LOVO/5-FU).
METHODS: After treatment with Ad-p53, LOVO/5-FU sensitivity to 5-Fu was investigated using tetrazolium dye assay. Multidrug resistance gene-1 (MDR1) gene expression was assayed by semi-quantitative reverse transcription-polymerase chain reaction and the expression of p53 protein was examined by Western blotting.
RESULTS: The reversal activity after treatment with wide-type p53 gene was increased up to 4.982 fold at 48 h. The expression of MDR1 gene decreased significantly after treatment with wide-type p53 gene, and the expression of p53 protein lasted for about 5 d, with a peak at 48 h, and began to decrease at 72 h.
CONCLUSION: Wide-type p53 gene has a remarkable reversal activity for the high expression of MDR1 gene in colorectal cancers. The reversal effects seem to be in a time dependent manner. It might have good prospects in clinical application.
