Precision medicine allergy immunoassay methods for assessing immunoglobulin E sensitization to aeroallergen molecules

Table 2 Allergen molecules of house dust and storage mites origin¹ used in singleplex and multiplex immunoassays[9,74,102,103]

Allergen molecule	Biological function, comments, CR
House dust mites (Dermatophagoides pteronyssinus, Dermatophagoides farinae, Euroglyphus maynei, Blomia tropicalis)
nDer p 1 rDer f 1	Cysteine-protease, cleavage of regulatory IgE synthesis CD23, CD25 Midgut (colon, intercolon, postcolon) and mite fecal pellets Group 1 major mite allergen, CR with nDer f 1
rDer p 2 nDer p 2 rEur m 2	Functional homologue of adaptor MD-2, TLR4 coreceptor Midgut (ventriculus, paired caeca), male reproductive tract, fecal pellets Group 2 major mite allergen, NPC2 family, CR Der p 2, Der f 2, homologue Lep d 2
rDer p 4	Group 4 major mite allergen
rDer p 5	Group 5 major mite allergen, homologue rBlo t 5
rDer p 7	LPS-binding protein, stimulation TLR2, group 7 mite allergen
rDer p 9	Serine protease, group 9 mite allergen
rDer p 10	Invertebrate panallergen tropomyosin muscular protein from mite locomotory muscles Group 10 CR mite allergen, invertebrate panallergen CR rAni s 3, nBla g 7, rPer a 7, rPen a 1, Pen m 1, Lit v 1, Hel as 1, Ven g 1, Uro du 1 Involved in house dust mites-crustaceans-mollusks syndrome
rDer p 11	Paramyosin, major allergen
rDer p 14	Apolipophorin-like allergen, lipid transport particles
rDer p 15 rDer p 18	Chitinase-like allergens
nDer p 20	Arginine kinase, CR shrimp Penaeus monodon Pen m 2
rDer p 21	Group 21 mite allergen, gut and fecal particles
rDer p 23	Peritrophin-like protein (8 kDa), group 23 mite major allergen Peritrophic lining of mite gut, fecal pellets
Storage mites (Glycyphagus domesticus, etc.)
rGly d 2	Group 2 mite allergens, NPC2 protein CR Lep d 2, homologue Der p 2 (limited CR)

¹n: Native purified;

r: Recombinant; Per a: Cockroach periplaneta americana allergen molecule; Mite Dermatophagoides pteronyssinus (Der p), Dermatophagoides farinae (Der f), Glycyphagus domesticus (Gly d), Lepidoglyphus destructor (Lep d), Blomia tropicalis (Blo t) allergen molecules; Nematode Anisakis simplex (Ani s) allergen molecule; Shrimp Penaeus aztecus (Pen a), Penaeus monodon (Pen m), Litopenaeus vannamei (Lit v 1), clam Venus gallina (Ven g), squid Uroteuthis duvauceli (Uro du) and snail Helix aspersa (Hel as) allergen molecules; CD: Cluster of Differentiation; CR: Cross-reactivity; LPS: Lipopolysaccharide; NPC2: Niemann-Pick type C2 protein; TLR: Toll-like receptor.