Basic Study
Copyright ©The Author(s) 2023.
World J Methodol. Dec 20, 2023; 13(5): 492-501
Published online Dec 20, 2023. doi: 10.5662/wjm.v13.i5.492
Figure 5
Figure 5 Effect of blood on performance of the exosome molecular assay. Urine samples derived from 4 donors were utilized, and blood or serum was spiked into urine before exosome purification. Levels of 9 mRNA targets were analyzed by quantitative polymerase chain reaction; the graph displays Ct values (y-axis). Conditions left to right: control urine specimens, processed following the standard protocol; urine specimens spiked with 1% blood and processed without additional centrifugation; urine specimens spiked with 1% blood and pre-processed following the standard protocol (centrifugation for 20 min at 2000 × g); urine specimens spiked with 1% serum and processed without additional centrifugation.