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©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Methodol. Sep 26, 2015; 5(3): 108-114
Published online Sep 26, 2015. doi: 10.5662/wjm.v5.i3.108
Published online Sep 26, 2015. doi: 10.5662/wjm.v5.i3.108
Hepatocyte selection medium eliminating induced pluripotent stem cells among primary human hepatocytes
Minoru Tomizawa, Department of Gastroenterology, National Hospital Organization Shimoshizu Hospital, Yotsukaido City, Chiba 284-0003, Japan
Fuminobu Shinozaki, Department of Radiology, National Hospital Organization Shimoshizu Hospital, Yotsukaido City, Chiba 284-0003, Japan
Yasufumi Motoyoshi, Department of Neurology, National Hospital Organization Shimoshizu Hospital, Yotsukaido City, Chiba 284-0003, Japan
Takao Sugiyama, Department of Rheumatology, National Hospital Organization Shimoshizu Hospital, Yotsukaido City, Chiba 284-0003, Japan
Shigenori Yamamoto, Department of Pediatrics, National Hospital Organization Shimoshizu Hospital, Yotsukaido City, Chiba 284-0003, Japan
Naoki Ishige, Department of Neurosurgery, National Hospital Organization Shimoshizu Hospital, Yotsukaido City, Chiba 284-0003, Japan
Author contributions: Tomizawa M and Shinozaki F wrote this manuscript; Motoyoshi Y, Sugiyama T, and Yamamoto S prepared figures; Ishige N supervised the manuscript.
Conflict-of-interest statement: Nothing to declare.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Minoru Tomizawa, MD, PhD, Department of Gastroenterology, National Hospital Organization Shimoshizu Hospital, 934-5 Shikawatashi, Yotsukaido City, Chiba 284-0003, Japan. nihminor-cib@umin.ac.jp
Telephone: +81-43-4222511 Fax: +81-43-4213007
Received: March 10, 2015
Peer-review started: March 18, 2015
First decision: May 13, 2015
Revised: May 22, 2015
Accepted: August 30, 2015
Article in press: August 31, 2015
Published online: September 26, 2015
Peer-review started: March 18, 2015
First decision: May 13, 2015
Revised: May 22, 2015
Accepted: August 30, 2015
Article in press: August 31, 2015
Published online: September 26, 2015
Core Tip
Core tip: Human induced pluripotent stem (hiPS) cells have the potential to differentiate into mature hepatocytes. If undifferentiated hiPS cells persist among transplanted hepatocytes, hiPS cells may potentially develop into cancer. Glucose and arginine are essential for the survival of most cells; however, mature hepatocytes survive in the media because they can produce glucose and arginine using galactokinase and ornithine transcarbamylase, respectively. Therefore, we created a hepatocyte selection medium (HSM) that lacks glucose and arginine but is supplemented with galactose and ornithine. After establishment of co-culture in HSM, human primary hepatocytes survive while hiPS cells die within three days.